| Chemical Biology of Secondary Metabolism

Recent publications


Evolution of fatty acid synthases

Fatty acid supplementation reveals differences in fatty acid metabolism. Published in Molecular Biosystems.


Advanced mechanistic "crosslinking"

ACP-enoyl reductase interaction. Published in JACS.


•   Sonnenschein, E.C., Pu, Y., Beld, J. and Burkart, M.D., Phosphopantetheinylation in the green microalgae Chlamydomonas reinhardtii; Journal of Applied Phycology, online (2016)


•   Beld, J., Abbriano, R., Hildebrand, M. and Burkart, M.D., Probing fatty acid biosynthesis using chemistry, in bacteria, cyanobacteria, green microalgae and diatoms; Molecular Biosystems, 10.1039/C5MB00804B (2016)


•   Uranga, C.C., Beld, J., Burkart, M.D., Mrse, A., Ghassemian, M.,  Córdova Guerrero, I. and Hernández Martínez, R. Fatty acid ester production in the trunk disease fungus Lasiodiplodia theobromae; Biochemical and Biophysical Research Communications, accepted online (2016)


•   Tallorin, L.T., Finzel, K., Beld, J., La Clair, J.J. and Burkart, M.D., Trapping of the Enoyl-Acyl Carrier Protein Reductase• Acyl Carrier Protein Interaction, Journal of the American Chemical Society, accepted online (2016)



•   Cahill, J. F., Darlington, T. K., Fizgerald, C., Shoepp, N., Beld, J., Burkart, M. D. and Prather, K. A., Editors' Highlight Previous Article Next Article Articles ASAP Online Analysis of Single Cyanobacteria and Algae Cells under Nitrogen-Limited Conditions Using Aerosol Time-of-Flight Mass Spectrometry; Analytical Chemistry, 2015, ASAP

•   Paz-Yepes, J., Beld, J., Burkart, M. D. and Palenik, B., Grazing on marine Synechococcus by new marine heterotrophic nanoflagellate isolates, 2015,submitted

•   Ferreira-Camargo, L., Tran, M., Beld, J., Burkart, M.D. and Mayfield, S. P., Selenocystamine improves protein accumulation in chloroplasts of eukaryotic green algae; AMB Express, 2015, online

•   Beld, J., Lee, D. J. and Burkart, M.D., Fatty acid biosynthesis revisited: structure elucidation and metabolic engineering, Molecular Biosystems, 2015


•   Beld, J., Cang, H., Burkart, M.D., Visualizing the chain-flipping mechanism in fatty acid biosynthesis, Angewandte Chemie, 2014

•   Beld, J., Finzel, K. and Burkart, M.D., Versatility of acyl-ACP synthetases Chemistry & Biology, 2014, highlighted in preview

till 2014

•   Beld, J., Sonnenschein, E.C., Vickery, R.V., Noel, J.P. and Burkart, M.D., The Phosphopantetheinyl Transferases: Catalysis of a Posttranslational Modification Crucial for Life, Natural Products Reports, 31, 61-108 (2014)
•   Bruegger, J., Caldara, G., Beld, J., Burkart, M.D. and Tsai, S.-C. Polyketide Synthase: Sequence, structure, function in Natural Products: Discourse, Diversity and Design, ISBN: 978-1-118-29806-0 (2014)
•   Bruegger, J., Haushalter, R., Vagstad, A., Beld, J., Shakya, G., Mih, N., Townsend, C.A., Burkart, M.D. and Tsai, S.-C. Probing the Selectivity and Protein-Protein Interactions of a Nonreducing Fungal Polyketide Synthase Using Mechanism-Based Crosslinkers. Chemistry & Biology 20, 1135-1146 (2013) see corrigendum
•   Beld, J., Blatti, J.L., Behnke, C., Mendez, M., and Burkart, M.D. Evolution of acyl-ACP thioesterases and β-ketoacyl-ACP synthases revealed by protein–protein interactions, Journal of Applied Phycology, 1-11 (2013)
•   Blatti, J.L., Beld, J., Behnke, C., Mendez, M., Mayfield, S.P. and Burkart, M.D., Manipulating fatty acid biosynthesis in microalgae through protein-protein interactions, PLoS One, 7, e42949 (2012)
•   Metanis, N., Foletti, C., Beld, J. and Hilvert, D. Rescue of kinetically trapped intermediates in oxidative protein folding by selenoglutathione, Israel Journal of Chemistry, 51, 953-959 (2011)
•   Metanis, N., Beld, J. and Hilvert, D. The chemistry of selenocysteine. Patai’s chemistry of functional groups, edited by Z. Rappoport (2011)
•   Beld, J. Woycechowsky, K. J., and Hilvert, D. Diselenides as universal oxidative folding catalysts of diverse proteins, Journal of Biotechnology, 150, 481-489 (2010)
•   Beld, J., Woycechowsky, K.J., and Hilvert, D. Small molecule diselenides catalyze oxidative protein folding in vivo, ACS Chemical Biology, 5, 177-182 (2009) cover art: “In contrast to disulfides, small amounts of exogenously added diselenide restore motility, and cells swim to the edge of soft agar plates.”
•   Beld, J., Woycechowsky, K.J., and Hilvert, D. Diselenide resins for oxidative protein folding, patent application EP09013216 (2009)
•   Coquiere, D., Bos, J., Beld, J. and Roelfes, G., Enantioselective artifical metalloenzymes based on a bPP Scaffold, Angewandte Chemie International Edition, 121, 6894-6897 (2009)
•   Beld, J., Woycechowsky, K. J., and Hilvert, D. Catalysis of oxidative protein folding by small-molecule diselenides, Biochemistry 47, 6985-6987 (2008)
•   Beld, J., Woycechowsky, K.J., and Hilvert, D. Selenocysteine as a probe of oxidative protein folding, Oxidative folding of Proteins and Peptides, edited by J. Buchner and L. Moroder, Royal Society of Chemistry (2008)
•   Steinmann, D., Nauser, T., Beld, J., Tanner, M., Günther, D., Bounds, P. L., Koppenol, W. H. Kinetics of tyrosyl radical reduction by selenocysteine, Biochemistry 47, 9602-9607 (2008)
•   Beld, J., Woycechowsky, K. J., and Hilvert, D. Selenoglutathione: Efficient oxidative protein folding by a diselenide, Biochemistry 46, 5382-5390 (2007)
•   Timmerman, P., Beld, J., Puijk, W. C., and Meloen, R. H. Rapid and quantitative cyclization of multiple peptide loops onto synthetic scaffolds for structural mimicry of protein surfaces, ChemBioChem 6, 821-824 (2005)
•   Basabe-Desmonts, L., Beld, J., Zimmerman, R. S., Hernando, J., Mela, P., García Parajo, M. F., Van Hulst, N. F., Van Den Berg, A., Reinhoudt, D. N., and Crego-Calama, M. A simple approach to sensor discovery and fabrication on self-assembled monolayers on glass, J. Am. Chem. Soc. 126, 7293-7299 (2004)
•   Timmerman, P., Beld, J., Meloen, R. H., and Puijk, W. C. Method for selecting a candidate drug compound, International patent WO-2004-077062 (2004)


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